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KMID : 0900920020260010033
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Korean journal of Animal Reproduction
2002 Volume.26 No. 1 p.33 ~ p.39
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Effect of Ca^{2+} Concentration in Fusion Medium on the Fusion, Nuclear Morphology and Development of Bovine Somatic Cell Nuclear Transfer Embryos
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Cho Je-Won
Kim Choung-Ik
Park Choon-Keun
Yang Boo-Keun
Cheong Hee-Tae
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Abstract
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This study was conducted to investigate the effect of Ca^{2+} concentration in fusion medium on the fusion, nuclear morphology and the development of bovine somatic cell nuclear transfer embryos. Bovine skin cells were transferred into an enucleated oocyte and fused with cytoplasm in the fusion medium containing with 0.05 to 1.0 mM Cacl_2. Nuclear transfer embryos were activated with a combination of A23187 and cycloheximide. Nuclear transfer embryos were fixed at 3 h after fusion or cultured for 7 ~8 days. Fusion rate was significantly (P＜0.01) increased by increasing the Ca^{2+} concentrations in the fusion medium from 0.05 mM (56.6%) to 0.5 mM (50.1%) and 1.0 mM (84.3%). More than 80% of reconstituted embryos underwent premature chromosome condensation (PCC) with 0.05, 0.1 mM CaCl_2, whereas 54.5% and 59.3% of embryos formed pronucleus (PN) directly without PCC in the 0.5 and 1.0 mM CaCl_2, groups. Blastocyst formation rates were significantly (P＜0.05) different between 0.1 mM and 1.0 mM CaCl_2groups. From the present result, it is suggested that the elevated Ca^{2+} concentrations in fusion medium can enhance the fusion and blastocyst formation rates of bovine nuclear transfer embryos.bryos.
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KEYWORD
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Somatic cell nuclear transfer, Ca^{2+} concentration, Fusion rate, In vitro development, Bovine
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